CHO Transient Transfection Kit01

CHO Trans Kit01 is a chemically defined reagent kit designed for high-density culture and transient transfection expression in CHO cells (primarily ExpiCHO-S). When used in combination, its components significantly enhance transfection efficiency and protein expression levels, offering excellent stability and reproducibility. This kit is suitable for research and large-scale biopharmaceutical production based on cell culture, but it is not intended for direct use in humans or as a drug.

Products & Product Performance Advantages

The optimized formulation of the basal medium supports high-density suspension cell growth.

Precise matching of cellular metabolic requirements significantly enhances protein expression levels.

Synergistic medium optimization improves the intracellular environment, increases cell tolerance to transfection, ensures proper protein folding and modification, and safeguards product bioactivity.

Utilizes cationic polymer technology for efficient plasmid DNA binding and delivery, boosting transfection efficiency by 30%-50% with simplified operation and high reproducibility.

Achieves live cell density exceeding 1.3 × 10⁷ cells/mL with ≥85% viability throughout the process

Employs high-quality domestic raw materials to substantially reduce R&D and production costs

Compatible with mainstream cell lines including CHO-K1, CHO-S, and multiple subclones, meeting both research and industrialization requirements.

CHO Series Product Introduction

The CHO media series includes one transient CHO CDM10 basal medium and three stable CHO CDM27, CHO CDM34, and CHO CDM35 basal media products. All are animal-free, protein-free, fully chemically defined media. The CHO media series is suitable for large-scale serum-free suspension culture of CHO cells and subsequent transfection expression. For therapeutic protein-related R&D and manufacturing processes, these media support high-density cell expansion and stable product expression. Specifically, CHO CDM27 is optimized for CHO-ZN cells, while CHO CDM34 and CHO CDM35 are tailored for CHOK1 and other CHO cell subtypes. To accommodate diverse culture systems and protein production workflows, complementary media products offer corresponding supplements and are also compatible with other commercial supplement formulations.